2025 Poster Session

Historical textiles found in burial sites or museums are often at risk of becoming stained and dirty, which can lead to chemical damage. Therefore, it is crucial to thoroughly clean these textiles to prevent irreversible harm and ensure the complete removal of any deposits without compromising the original material. Scientific advancements and technology have innovated techniques that have been developed for the bio-cleansing of historic textiles and artistic surfaces using enzymes. This study specifically focuses on the application of lipase and alpha-amylase, which have been immobilized onto chitosan nanoparticles for the purpose of cleaning historical textiles. The synthesis, activation, and immobilization of the nanoparticles were evaluated and characterized using FT-IR and SEM, and the optimal loading time and units for the two enzymes were determined. It was found that the ideal duration for amylase was 4 hours with 25 units, while for lipase, it was 3 hours with 15 units.The cleaning procedure encompasses several sequential steps. It commences with the elimination of any prior restoration, followed by the initial installation process. Subsequently, the object undergoes mechanical cleansing.Within this study, chitosan was employed for the covalent immobilization of α-amylase and lipase enzymes, which were subsequently utilized for the cleansing and restoration of vintage textiles. The optimal loading units and durations were determined for both enzymes. Nanoparticle preparation, activation, and enzyme immobilization were evaluated using FT-IR, SEM, and TGA instrumentation.This approach ensures the preservation of the antique fabric's hue and serves as a deterrent against further fabric deterioration. The mending process encompasses multiple sequential steps, with the initial step entailing the removal of any previous restoration.